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puc gw amp r t5 buga  (Azenta)
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Azenta puc gw amp r t5 buga
( A ) EOP assay of WT and yjbI mutant cells on PYE with vancomycin at 7.5 (VAN 7.5 ), 20 (VAN 20 ), or bacitracin (3 µg/mL, BAC 3 ). Plates were incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . ( B – D ) β-galactosidase assay measurement from WT and mutant cells harboring the pP bugA - lacZ reporter plasmid with or without inductions for 2 h ( B , D ) or 4 h ( C ) with CuSO4 or ZnSO4 (80 µM). All β-galactosidase measurements are reported relative to the 100% activity level of the uninduced state in WT cells. Error bars are defined as +/− standard deviation. For ( D ), cells were grown in PYE containing 0.3% xylose, and for ( B , C ) without xylose. ( E ) EOP assay of WT and cells ectopically expressing BugA from the constitutive E. coli phage T5 promoter at the bugA locus ( bugA :: T5- bugA ) or from a replicative plasmid (pMT464- bugA ). Cells were spotted on PYE plates with or without BAC 3 and incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . .
Puc Gw Amp R T5 Buga, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) EOP assay of WT and yjbI mutant cells on PYE with vancomycin at 7.5 (VAN 7.5 ), 20 (VAN 20 ), or bacitracin (3 µg/mL, BAC 3 ). Plates were incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . ( B – D ) β-galactosidase assay measurement from WT and mutant cells harboring the pP bugA - lacZ reporter plasmid with or without inductions for 2 h ( B , D ) or 4 h ( C ) with CuSO4 or ZnSO4 (80 µM). All β-galactosidase measurements are reported relative to the 100% activity level of the uninduced state in WT cells. Error bars are defined as +/− standard deviation. For ( D ), cells were grown in PYE containing 0.3% xylose, and for ( B , C ) without xylose. ( E ) EOP assay of WT and cells ectopically expressing BugA from the constitutive E. coli phage T5 promoter at the bugA locus ( bugA :: T5- bugA ) or from a replicative plasmid (pMT464- bugA ). Cells were spotted on PYE plates with or without BAC 3 and incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . .

Journal: The EMBO Journal

Article Title: Asymmetric envelope surface disposition of secreted protein YjbI controls bimodal antibiotic susceptibilities in C. crescentus

doi: 10.1038/s44318-025-00668-x

Figure Lengend Snippet: ( A ) EOP assay of WT and yjbI mutant cells on PYE with vancomycin at 7.5 (VAN 7.5 ), 20 (VAN 20 ), or bacitracin (3 µg/mL, BAC 3 ). Plates were incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . ( B – D ) β-galactosidase assay measurement from WT and mutant cells harboring the pP bugA - lacZ reporter plasmid with or without inductions for 2 h ( B , D ) or 4 h ( C ) with CuSO4 or ZnSO4 (80 µM). All β-galactosidase measurements are reported relative to the 100% activity level of the uninduced state in WT cells. Error bars are defined as +/− standard deviation. For ( D ), cells were grown in PYE containing 0.3% xylose, and for ( B , C ) without xylose. ( E ) EOP assay of WT and cells ectopically expressing BugA from the constitutive E. coli phage T5 promoter at the bugA locus ( bugA :: T5- bugA ) or from a replicative plasmid (pMT464- bugA ). Cells were spotted on PYE plates with or without BAC 3 and incubated for 2 days at 30 °C. The comparison is to growth on PYE without treatment, as in Fig. . .

Article Snippet: pUC-GW-Amp R T5-bugA , Genewiz/Dataset , 5’-fragment of bugA fused to T5 promoter.

Techniques: Mutagenesis, Incubation, Comparison, Plasmid Preparation, Activity Assay, Standard Deviation, Expressing